DSmRT Exome Capture Technology
Exome sequencing is a powerful tool for surveying genomes especially where the genome size is too large or high read depth is essential for downstream bioinformatic analysis. Traditional Exome sequencing methods are costly and require annotated genome to synthesize capture probes. In our patented Dual-strand Specific mRNA Targeted (DSmRT) technology we create biotinylated exome capture probes directly from the mRNA of the species. These probes are then used to enrich the protein coding regions of genes for both exome and bisulfite sequencing apllications. DSmRT Exome-Seq technology is available as a service through SAHMRI’s Genomics Facility.
Advantages of the DSmRT technology:
1.Fast and very affordable (80-90% cheaper) when compared to commercial products.
2.Enables Exome Sequencing for any species with or without a reference genome.
3.Probes are produced directly from RNA/mRNA.
4.Enables simultaneous methylation profiling via bisulfite sequencing.
5.Highly specific probes.
Gene fusion calling
Take the advantage of DSmRT technology to look into structural variations (SVs) and gene fusions. DSmRT technology combines the advantages of RNA-Seq, Exome-Seq and bisulfite-Seq into one solution. DSmRT technology enables high read depth coverage with simultaneous exome and methylation profiling to confidently call for chromosomal rearrangements and to identify novel fusion genes. For disease research (eg. cancer) don’t miss out on important information for cohort-specific, novel fusion genes which may be missing from commercial exome and targeted bisulfite sequencing solutions.
Exome Sequencing for Variant calling
DSmRT technology enables Exome Sequencing for any species with or without a reference genome. DSmRT exome sequencing is proven to be highly specific and efficient in capturing the exome across many sample types.
Targeted bisulfite sequencing
As the DSmRT exome capture probes target both strands of DNA (sense or antisense) the technology enables methylation profiling of exonic regions (exome) along with UTR/intron boundaries via DSmRT bisulfite sequencing.
Annotating transcriptome reference genomes
RNA sequencing has been used for creating transcriptome reference genomes which normally lacks information regarding exon-intron boundaries. DSmRT exome sequencing could be combined with RNA sequencing to create transcriptome reference genomes to precisely annotate exon-intron boundaries.
DNA barcoding of species for biodiversity and exploration projects
If you are working on barcoding/classification of tens of species (e.g. exploration sites or biodiversity studies), take the advantage of affordable DSmRT technology to capture the information for the most important regions of the genome across all samples. DSmRT technology is fast, affordable, highly accurate and scalable to accommodate your project’s needs.
We have 2 levels of services for DSmRT technology:
We synthesize the DSmRT exome capture probes, perform library preparations for DSmRT exome-seq and/or DSmRT bisulfite sequencing, and sequence the libraries on the Illumina sequencing platform of your choice (e.g. MiSeq, NextSeq or NovaSeq) depending of the required coverage/depth and the number of samples.
As per the basic service, plus a full bioinformatic analysis.