Illumina Sequencing

The facility has three next generation sequencers which use Illumina’s SBS chemistry – see the Equipment pages for more details of the machines. The Hiseq 2500 is running v4 chemistry which is capable of up to 2 x 125 bp paired-end reads and 250 million reads per lane. For projects where 8 lanes are required (ie. a whole sequencing flow-cell), the read length strategy can be quite flexible.

The facility offers both a sequencing-only service and one with complete library generation for the common applications.