SAHMRI Genomics Genomics

  • Getting Started
    • Getting Started
    • Frequently Asked Questions
    • Sample Submission
  • Services
    • Illumina Sequencing
    • Library Preparation
    • RNA / DNA Assesment
  • Equipment
    • Illumina Novaseq 6000
    • Illumina Nextseq 500
    • Illumina Miseq
    • 10X Genomics Chromium
    • Oxford Nanopore Minion
    • Applied Biosystems Quantstudio 7
    • Biorad CFX Connect Real-Time System
    • Covaris M220
    • Agilent 2100 Bioanalyzer and 2200 Tapestation, PE Labchip
  • Contact

RNA / DNA Assesment

Starting material of the highest practical quality is important to ensure the best data is generated. Nanodrop (UV/Vis) measurements are good for assessing sample purity and estimating concentration, however, analysis by microfluidic devices (eg. Agilent Tapestation) are ideal for assessing RNA and DNA size and quality due to their high resolution and low sample usage.

All samples and libraries are required to be assessed prior to sequencing; the facility is able to perform this assessment at a reasonable cost for you if you don’t have access to these instruments. Where input concentrations are critical (or low by Nanodrop) a fluorescence measurement should be performed with an appropriate dye and reader (eg. Qubit) for accuracy. The table below shows the input requirements for some of the more commonly used Agilent sample assays.

AGILENT MICROFLUIDIC ASSAYS

RNA/DNA Assessment Sample Type No. Samples / run Input (ng/uL) Supply (uL)
Agilent Tapestation High Sensitivity D1000 Screentape DNA (50 - 1000 bp) 2 - 16 0.005 - 0.5 3
Agilent Tapestation RNA Screentape Total RNA 2 - 16 50 - 500 3
Agilent Bioanalyzer RNA Picochip Total RNA up to 11 0.1 - 3 3
Agilent Tapestation Genomic DNA DNA (200 - 60000 bp) 2 - 16 5 - 300 3

  • Illumina Sequencing
  • Library Preparation
  • RNA / DNA Assesment

Contact
Genomics at SAHMRI
North Terrace, Adelaide, SA 5000

08 8128 4330
genomics@sahmri.com

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